The following is a list of missions in Marvel's Spider-Man. Missions serve as the main storyline of the game, indicated by yellow icons () on the map, and generally begin with a cutscene. Many missions will result in a reward of some kind, such as a new gadget or suit, in addition to experience points. There are 44 missions available in the base game, with 26 additional missions featured in The City That Never Sleeps downloadable content.
The City That Never Sleeps
|Trophy: Friendly Neighborhood Spider-Man|
|Complete all Side Missions|
Each district has different side missions that players can complete. These appear as blue icons () on the map and are optional missions provided to the player as they enter or unlock specific regions. Some side missions are unlocked through story progression while others take a bit more work. They are typically assigned to Spider-Man by the citizens of New York City, and award experience points upon completion.
As players progress through the game, citywide activities are unlocked after completing certain missions. These are optional activities that are scattered throughout the city. Completing them will reward players with tokens, collections and in some cases, suits. Some side missions require certain activities to be completed in order to be unlocked, as seen in The City That Never Sleeps campaign.